Table 1 Buffers, doses, and proteins used with each colony.

From: A new artificial diet for western corn rootworm larvae is compatible with and detects resistance to all current Bt toxins

Protein

Diet

Colony

Protein Buffer

Dose (µg/cm2)

Dose 1

Dose 2

Dose 3

Dose 4

Dose 5

Dose 6

Dose 7

Dose 8

Cry34/35Ab1

Proprietary Diet A

Brookings-ND

10 mM Sodium Citrate pH 3.5

0.00

0.08

0.24

0.74

2.22

6.67

20.00

60.00

WCRMO-1

Brookings-ND

0.00

0.09

0.28

0.85

2.55

7.65

22.94

68.82

DAS-59122-7-S1 (6 gen.)

0.00

0.09

0.28

0.85

2.55

7.65

22.94

68.82

DAS-59122-7-S2 (18 gen.)

0.00

0.09

0.28

0.85

2.55

7.65

22.94

68.82

Cry3Bb1

Proprietary Diet B

Brookings-ND

10 mM Sodium Carbonate Bicarbonate pH 10

0.00

22.19

44.29

88.57

177.14

.

.

.

WCRMO-1

Brookings-ND

0.00

24.54

48.97

97.95

195.89

.

.

.

MON88017-S1 (11 gen.)

0.00

24.54

48.97

97.95

195.89

391.79

.

.

MON88017-S2 (11 gen.)

0.00

24.54

48.97

97.95

195.89

391.79

.

.

mCry3A

Proprietary Diet C

Brookings-ND

0.00

0.34

1.03

3.10

9.29

27.87

.

.

WCRMO-1

Brookings-ND

0.00

0.34

1.03

3.10

9.29

27.87

.

.

MIR604-S (50 + gen.)

0.00

0.30

0.90

2.70

8.10

24.30

.

.

eCry3.1Ab

Proprietary Diet C

Brookings-ND

0.00

0.34

1.03

3.10

9.29

27.87

.

.

WCRMO-1

Brookings-ND

0.00

0.34

1.03

3.10

9.29

27.87

.

.

5307-S (35 gen.)

0.00

0.30

0.90

2.70

8.10

24.30

.

.

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