Figure 8
The CaMKIIβH and βeH variants can still co-assemble with the full-length CaMKIIα hub domain, as indicated by FRET assays and nuclear exclusion. (a) FRET-imaging of Cos-7 cells co-expressing an mCherry-labelled CaMKIIα hub domain with various GFP-CaMKII constructs; the shown line-scans were taken as indicated in the mCherry images. Robust corrected FRET (FRETC) was observed for all constructs, except for the truncated negative control 1–316, which completely lacks the association domain. (b) Quantification of the FRET signals in the cytoplasm and the nucleus. No significant differences were detected, expect for the truncated 1–316 negative control. (c) Quantification of nuclear localization revealed that co-expression with the CaMKIIα hub domain prevented any increase in nuclear localization for the βH and βeH variants. The only construct with increased nuclear localization was the truncated monomeric 1–316.
