Figure 4
Effect of AMPKα inhibition on TRAIL-BBR-induced apoptosis. (A) C42 cells overexpressing empty vector (C42-EV) or FLAG epitope-tagged dominant negative AMPKα1 vector (C42-DN) were treated with DMSO or TRAIL alone or TRAIL in combination with increasing concentrations of BBR for 8 hrs. The samples were analyzed by Western blot analyses with the indicated antibodies. (B) C42 cells were transiently transfected with 50 nM of either control siRNA (lanes 1 & 2), or AMPKα1 siRNA (lanes 3 & 4) or AMPKα2 siRNA (lanes 5 & 6) for 72 hrs and then treated with DMSO or a combination of TRAIL and BBR for 8 hrs. Western blot analysis was performed utilizing the antibodies indicated. For all western blots (A,B), the bands from adjacent lanes were cropped from the same blot (full-length uncropped blots are included in Supplementary Fig. S6).
