Figure 7
Role of DR4 and DR5 in mediating TRAIL-BBR-induced apoptosis. Subconfluent populations of C42 (A) or Huh7 (B) cells were transiently transfected with 50 nM of either control siRNA (lanes 1 & 2), or DR4 siRNA (lanes 3 & 4) or DR5 siRNA (lanes 5 & 6) or both DR4 and DR5 siRNA (lanes 7 & 8) followed by treatment with DMSO or a combination of TRAIL (100ng/ml) and BBR (50 µM) for 6 hrs. The samples were analyzed by Western blots with the indicated antibodies. For all western blots (A,B), the bands from adjacent lanes were cropped from the same blot (full-length uncropped blots are included in Supplementary Fig. S7).
