Figure 6
From: GLYI and D-LDH play key role in methylglyoxal detoxification and abiotic stress tolerance
Loss of function study reveals role of different genes in MG detoxification: BY4741 wild type yeast cells, GLYIKO, GLYIIKO and D-LDHKO mutant yeast cells containing empty vector pYES2 were grown on Ura− SD agar plates with different supplements to check for their contribution to MG detoxification. (i) Pictorial representation of various strains used. (ii) Medium supplemented with 20% glucose. (iii) medium supplemented with 20% glucose and 1 mM MG. (iv) Medium supplemented with 20% galactose. (v) Medium supplemented with 20% galactose and 1 mM MG. (vi) Medium supplemented with 20% galactose and 2 mM MG. (vii) Medium supplemented with 20% galactose and 3 mM MG. (viii) Medium supplemented with 20% galactose and 4 mM MG.
