Figure 3 | Scientific Reports

Figure 3

From: Physiological and transcriptome changes induced by Pseudomonas putida acquisition of an integrative and conjugative element

Figure 3The alternative text for this image may have been generated using AI.

Differential expression of the Pspu28 phage P1548 promoter at single-cell level in P. putida with or without ICEclc. (A) Representative micrographs of P. putida strains carrying single-copy chromosomal inserts of the P1548-mcherry and the ICEclc bistable Pint–promoter –egfp fusions, via mini-transposon delivery. Images show merged phase–contrast (grey), eGFP (green), and mCherry (magenta). Scale bar indicates 5 μm. (B) Scatter plots showing mCherry (from P1548) and eGFP (from Pint) fluorescence intensities among individual cells (circles) of P. putida without (strain 180) and with ICEclc (strain 172) at exponential and late stationary phases in MM with 3CBA. Threshold (dashed line) between Pint-active and -inactive cells of strain 172 at late stationary phase was calculated according to Reinhard and van der Meer71. Note that mCherry and eGFP fluorescence among the Pint-active cells is not correlated significantly (R2 = 0.00877). (C) Box plots showing mCherry (from P1548) fluorescence intensity among individual cells of P. putida without (strain 180, 181, and 177) and with ICEclc (strain 172, 166, and 169) at exponential and late stationary phases in MM with 3CBA. The number of measured cells is indicated for every sample. Note that the different P. putida strains have randomly inserted fluorescence reporter constructs.

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