Figure 6
Average real-time PCR threshold cycle (CT) values for amplification of the Chlamydiae 16 S rRNA gene. (a) Recorded CT values for positive samples among children and adults. The children case group showed significantly lower CT values compared to the children control group and adults case and control groups. (b) Recorded CT values for 16 S rRNA sequences classified as families in the phylum Chlamydiae, C. trachomatis (CT), and non-trachomatis Chlamydiaceae (NTC). CT values were significantly lower for the Chlamydiaceae family compared to Parachlamydiaceae, Simkaniaceae, and Criblamydiaceae families. CT values for positive samples for C. trachomatis were recorded as significantly lower than those for NTC positive samples. (c) Recorded CT values for 16 S rRNA sequences classified as families in the phylum Chlamydiae, CT, and NTC among children. (d) Recorded CT values for 16 S rRNA sequences classified as families in the phylum Chlamydiae, CT, and NTC among adults. The statistical significance is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
