Figure 4 | Scientific Reports

Figure 4

From: Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells

Figure 4

Winter bear serum impacts main factors of autophagy/lysosomal degradation in human muscle cells. Illustrative immunodetection (A) and corresponding quantification (B) of SQSTM1 (p62) and the LC3b II/I ratio in human myotubes exposed for 24 hours to SBS or WBS (C). Myotube expression of CTSL and ATG3 was measured by RT-qPCR, normalized against TBP mRNA levels, and expressed as a fold change relative to the SBS condition (D). Data are the mean ± SEM of at least 3 independent experiments. Significant differences result from paired T-tests (*p < 0.05; **p < 0.01).

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