Figure 3 | Scientific Reports

Figure 3

From: Cyperenoic acid suppresses osteoclast differentiation and delays bone loss in a senile osteoporosis mouse model by inhibiting non-canonical NF-κB pathway

Figure 3

Effect of cyperenoic acid on expression of osteoclast-related genes. BMs were pre-treated with M-CSF with DMSO and left unstimulated (open bars), M-CSF with DMSO for 30 min, followed by RANKL stimulation (closed bars) or M-CSF with cyperenoic acid (100 µM) for 30 min, followed by RANKL stimulation (hatched bars) for indicated times. Total RNA was extracted and subjected to RT-qPCR. Osteoclast-related genes were nfatc1 (A), ctsk (B), irf8 (C), acp5 (D) and cfos (E). The data are representative of three independent experiments and presented as the mean ± S.D. ***p < 0.001, **p < 0.01 and *p < 0.05 indicated statistical significance.

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