Figure 4 | Scientific Reports

Figure 4

From: Cyperenoic acid suppresses osteoclast differentiation and delays bone loss in a senile osteoporosis mouse model by inhibiting non-canonical NF-κB pathway

Figure 4

Effect of cyperenoic acid on activation of MAPK, canonical or non-canonical NF-κB pathways and expression of NFATc1 and c-Fos. M-CSF treated BMs were pre-treated with cyperenoic acid (100 μM) or vehicle control DMSO for 30 min and RANKL (100 ng/ml) was used to stimulate cells at the indicated times. Whole cell lysates were analyzed for phosphorylation forms of MAPK and canonical NF-κB (A) or non-canonical NF-κB (B) by Western blot. (C–D) The quantitative band density of phosphorylated p100 and total p52 were normalized by total p100 or β-actin, respectively. (E) The level of c-Fos and NFATc1 were analyzed by Western blot. β-actin was used as a loading control. Data were representative of three independent experiments.

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