Figure 6 | Scientific Reports

Figure 6

From: Cyperenoic acid suppresses osteoclast differentiation and delays bone loss in a senile osteoporosis mouse model by inhibiting non-canonical NF-κB pathway

Figure 6

Effect of cyperenoic acid on bone resorption by mature osteoclast. (A) A procedure used to evaluate the effect of cyperenoic acid on bone resorption by RANKL-induced osteoclasts on bone slices is shown. (B) The resorption areas were stained with toluidine blue and the resorption areas were measured and quantitated as a percentage of total resorbed area using Image-J software at 100-fold magnification of at least three unrelated fields. (C–E) The staining of bone resorption areas on bone slices were shown. The data presented as the mean ± S.D. ***p < 0.001 and *p < 0.05 indicated statistical significance.

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