Figure 3 | Scientific Reports

Figure 3

From: Virus-encoded miRNAs in Ebola virus disease

Figure 3

EBOV-encoded miRNAs were detectable in the plasma of EBOV/Kikwit-infected NHPs. Ten candidate miRNAs were detected by 19 LNA primer sets in rhesus macaque plasma from an EBOV/Kikwit infection cohort (NHP n = 3). Data are presented as the mean ± SD of biological replicates run in technical triplicate. miRNAs were assayed with custom LNA primer sets obtained from Exiqon, Inc. following manufacturer’s instructions (see Methods for details). Each miRNA target was amplified by two separate primer sets. Viral load was determined using an RT-qPCR assay which amplifies part of the viral glycoprotein sequence.

Back to article page