Figure 1 | Scientific Reports

Figure 1

From: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

Figure 1

Fluorescence signal from VZV62 dT-FAM probes. (A) Signal profile obtained after converting the Outer (VZV62F3), Inner (VZV62FIP) and Loop (VZV62LPB) primers into dT-FAM fluorescent probes. Input template concentration pVZV-ORF62 plasmid: 1 × 106 copies/µL. (B) Variation of fluorescence signal intensity due to varying levels of the Base Mix ranging from 0.5X, 1.0X, 1.5X, 2.0X and 2.5X. Input template concentration pVZV-ORF62 plasmid: 1 × 106 copies/µL. Water was used as a negative control. Each Time[30 sec interval] unit (X-axis) represents 30 seconds on the ESEQuant instrument.

Back to article page