Figure 2 | Scientific Reports

Figure 2

From: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

Figure 2

Optimization of signal intensity using the VZV62LPB-FAM probe. (A) Effect of increasing levels of F3/B3 primer pair from 0.5 µM (Mix 5), 1.0 µM (Mix 6) and 2.0 µM (Mix 7OPT). (B) Variation in signal intensity with increasing levels of the VZV62LPB-FAM probe from 0.1 µM, 0.2 µM and 0.3 µM. Input template concentration of pVZV-ORF62 plasmid: 1 × 106 copies/µL. Each Time[30 sec interval] unit (X-axis) represents 30 seconds on the ESEQuant instrument.

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