Figure 4 | Scientific Reports

Figure 4

From: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

Figure 4

Schematic depiction of the binding of VZV62LPB-FAM probe to its target. The FAM fluorophore attached to the VZV62LPB-FAM probe is self-quenched in unbound state. Post binding to the dumbbell shaped DNA target, the FAM fluorophore is de-quenched resulting in fluorescence development. For simplicity, only one dumbbell which would bind to the LPB loop primer (VZV62LPB) is shown in this depiction.

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