Table 4 Comparison of limit of detection of the VZV62 FLOS-LAMP with intercalating dye based LAMP assay. nd = not detected.

From: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

pVZV-ORF62

(copies/µL)

Copies/Reaction (20 µL)

Time to positivity (Tp)

Flos-Lamp Probe

Intercalating Dye

1.0 × 105

5,00,000

7.5 min

12.5 min

1.0 × 104

5,00,00

8.5 min

14.0 min

1.0 × 103

5,000

10.0 min

15.5 min

1.0 × 102

500

14.5 min

nd

1.0 × 101

50

nd

nd

Back to article page