Figure 2

Ammoniagenic and gluconeogenic enzymes induced by metabolic acidosis are not changed by Nrf2 deficiency. WT and Nrf2 KO mice were fed a normal diet or a HCl-containing standard diet for 7 days. (a) qPCR analysis of Gls in the kidney. (b) Immunoblot detection of GLS using 40 µg total kidney homogenate. (c). qPCR analysis of Pepck mRNA expression in the kidney (d). Immunoblot detection of PEPCK using 40 µg total kidney homogenate. mRNA values were normalized to Ppia and β-TUBULIN was used as a house-keeping control in immunoblots. Data represent mean ± S.D. of n = 6 animals per group. Statistical analysis was performed with a one-way analysis of variance (with Tukey’s post test) for mRNA data and Student’s t-test comparing Nrf2 KO and WT for protein data; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.