Figure 6
From: NRF2 regulates the glutamine transporter Slc38a3 (SNAT3) in kidney in response to metabolic acidosis
Metabolic acidosis increases oxidative stress in Nrf2 KO kidney. WT and Nrf2 KO mice were fed a normal diet or a HCl-containing standard diet for 7 days. qPCR analysis of the rate limiting glutathione synthesis enzyme, GSL, subunits (a) Gclc and (b) Gclm. (c) total glutathione contents of kidney tissue, normalized to tissue weight. qPCR analysis of kidney proximal tubule oxidative stress markers (d), Kim1 and (e), Car3. mRNA values were normalized to Ppia. Data represent mean ± S.D. of n = 6 animals per group. Statistical analysis was performed with a one-way analysis of variance (with Tukey’s post test); *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.
