Table 2 Weight, food intake and urinary values in C57Bl6 and Nrf2 KO mice under normal diet and a 2 or 7 days HCl load
From: NRF2 regulates the glutamine transporter Slc38a3 (SNAT3) in kidney in response to metabolic acidosis
Baseline | 2 days HCl | 7 days HCl | ||||
|---|---|---|---|---|---|---|
WT N = 12 | Nrf2 KO N = 12 | WT N = 6 | Nrf2 KO N = 6 | WT N = 6 | Nrf2 KO N = 6 | |
Weight (g) | 25.1 ± 1.9 | 23.0 ± 0.6* | 26.0 ± 1.1 | 24.6 ± 0.7* | 24.2 ± 0.9# | 22.7 ± 1.1# |
Food intake (g/24 h/body weight) | 0.30 ± 0.06 | 0.40 ± 0.06 * | 0.32 ± 0.05 | 0.34 ± 0.04 | 0.29 ± 0.01 | 0.30 ± 0.03# |
Water intake (ml/24 h) | 2.2 ± 1.1 | 1.5 ± 0.7* | 2.5 ± 1.7 | 2.1 ± 0.9 | 4.8 ± 2.6# | 5.5 ± 2.6# |
Urine | ||||||
Volume (ml/24 h) | 2.4 ± 0.5 | 1.8 ± 0.5* | 2.3 ± 0.7 | 2.5 ± 0.3# | 2.1 ± 0.8 | 2.8 ± 0.9# |
Creatinine excretion (µmol/24 h) | 6.4 ± 0.7 | 5.7 ± 1.2 | 6.3 ± 1.2 | 6.8 ± 0.9 | 4.6 ± 1.9 | 6.5 ± 1.0 |
Urinary pH | 6.3 ± 0.2 | 5.9 ± 0.3* | 5.6 ± 0.1 | 5.4 ± 0.1 | 5.6 ± 0.5 | 5.3 ± 0.1 |
NH4/Crea (mEq/mmol) | 11.8 ± 12.3(6) | 22.9 ± 16.2*(6) | 71.5 ± 7.4# | 78.7 ± 13.8# | 140.2 ± 14.5# | 133.7 ± 23.9# |
TA/Crea (mEq/mmol) | 14.9 ± 2.8(6) | 22.4 ± 4.7*(6) | ND | ND | 9.0 ± 5.1# | 14.0 ± 0.8*# |
NAE/Crea (mEq/mmol) | 26.5 ± 9.9(6) | 50.1 ± 14.8*(6) | ND | ND | 149.1 ± 10.7 | 147.7 ± 23.7 |
Pi/Crea (mEq/mmol) | 7.5 ± 0.6 | 8.8 ± 1.0* | 22.7 ± 4.2# | 24.2 ± 3.6# | 2.3 ± 0.6# | 3.4 ± 0.5*# |
Ca2+/Crea (mEq/mmol) | 0.19 ± 0.4 | 0.29 ± 0.08* | ND | ND | 3.4 ± 1.0# | 3.8 ± 1.2# |
Na+/Crea (mEq/mmol) | 25.9 ± 2.7 | 27.7 ± 6.3 | ND | ND | 35.5 ± 3.5# | 41.0 ± 3.8*# |
Cl−/Crea (mEq/mmol) | 48.8 ± 7.0 | 50.4 ± 10.0 | ND | ND | 213.7 ± 15.7# | 209.2 ± 25.4# |
K+/Crea (mEq/mmol) | 69.7 ± 9.4 | 70.2 ± 10.0 | ND | ND | 78.6 ± 6.2 | 77.1 ± 7.2 |
