Figure 8

Predicted functional metagenomes of in caecal digesta (A) and mucosa (B) microbiota of pigs fed the control (CON) and flaxseed meal (FM) diets. Linear discriminant analysis (LDA) was performed to identify significant changes in the proportion of reconstructed functional pathways obtained from PICRUSt predictive algorithms at Kyoto Encyclopaedia of Genes and Genomes (KEGG; level 2 and 3). Analysis was performed using linear discriminant analysis of effect size (LEfSe), a metagenome analysis approach which performs the LDA following the Wilcoxon Mann-Whitney test to assess effect size of each differentially abundant variable. Colour code represents the class of treatment. Red indicates variables that were detected as significantly (Log LDA > 2.00) more abundant in CON, whereas green indicates variables that were detected as significantly more abundant in FM diet-fed pigs. Blue indicates variables that were detected as significantly more abundant in OH-diet fed pigs.