Figure 6 | Scientific Reports

Figure 6

From: Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes

Figure 6

PEX2 interacts with PMP70. (A) Co-immunoprecipitation of HeLa cells transfected with PEX2-mCitrine or PEX13-mGFP. Cell lysate was immunoprecipitated with anti-GFP nanobody. The immunoprecipitate were analyzed by anti-GFP and anti-PMP70 antibodies. IP, immunoprecipitation; WB, Western Blot. (B) STED raw images of HeLa cells co-transfected with PEX2-mCitrine and TagRFP-PEX13 immunolabeled with GFP and RFP nanobodies conjugated to Atto647N and Atto594, respectively. Scale bar 500 nm. (C) Quantitative Western blot analysis of PMP70 abundance in HeLa cell lysates transfected with GFP empty vector and PEX2-mCitirine. Differences were not statistically significant as measured by one-way ANOVA: p < 0.05. N = 3.

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