Figure 2 | Scientific Reports

Figure 2

From: The C-terminal HRET sequence of Kv1.3 regulates gating rather than targeting of Kv1.3 to the plasma membrane

Figure 2

Current of A413V truncated mutants in HEK and CHO cells. (A) Representative current traces recorded in HEK transfected with the EGFP-tagged A413V-ΔC or the A413V-NOHRET construct (arrows). Currents were recorded upon 30-ms-long depolarizations to + 50 mV from a holding potential of −120 mV. (B) Representative current traces recorded in CHO transfected with the EGFP-tagged A413V-ΔC or the A413V-NOHRET construct. Recording conditions were the same as in panel A. (C,D) Box plot of the current density measured in HEK (C) or CHO (D) transfected with the full length (A413V-FL), A413V-ΔC or A413V-NOHRET constructs. The data are reported as mean (dashed line), median (solid line), first (top box) and third quartiles (bottom box), and maximum and minimum. Current density was determined as detailed in the materials and methods.

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