Table 1 Peptide mapping shows that the AIEX step elution protocol enriched for EVs in peak 2.

From: Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography

Top 50 Exosome markers from exocarta

Gene Symbol

Enriched in peak:

Gene Symbol

Enriched in peak:

CD9

2

VCP

 

HSPA8

2

TPI1

2

PDCD6IP

2

PPIA

2

GAPDH

1

MSN

1

ACTB

 

CFL1

 

ANXA2

2

PRDX1

2

CD63

2

PFN1

2

SDCBP

2

RAP1B

2

ENO1

 

ITGB1

2

HSP90AA1

 

HSPA5

 

TSG101

 

SLC3A2

1

PKM

2

HIST1H4A

1

LDHA

2

GNB2

2

EEF1A1

2

ATP1A1

2

YWHAZ

2

YWHAQ

2

PGK1

2

FLOT1

 

EEF2

 

FLNA

 

ALDOA

2

CLIC1

 

HSP90AB1

1

CDC42

2

ANXA5

2

CCT2

1

FASN

 

A2M

 

YWHAE

2

YWHAG

2

CLTC

 

TUBA1B

 

CD81

2

RAC1

2

ALB

1

LGALS3BP

 
  1. Peak 1 and peak 2 were lysed in 6 M urea and subjected to peptide mapping. The top 50 proteins reported to be found in exosomes on exocarta32 were then used to determine whether the majority of EV proteins resided in peak 1 or in peak 2 n = 3. ANOVA calculated by Waters progenesis software. Proteins with a p value of 0.05 or less were included in this subsequent analysis.
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