Figure 5 | Scientific Reports

Figure 5

From: Bacterial Pore-Forming Toxins Promote the Activation of Caspases in Parallel to Necroptosis to Enhance Alarmin Release and Inflammation During Pneumonia

Figure 5

Caspase-2 and -11 KO reduces PFT-mediated inflammation during bacterial pneumonia. (a) Hematoxylin and Eosin stain of mice genetically deficient in MLKL, caspase-2 and caspase-4 experiencing Sma pneumonia or mock challenged with PBS. (b) Representative IF staining of lungs of caspase-2 KO mice infected with Sma, cell nucleus (DAPI, Blue), Ly6G (red). (c) Levels of IL-1α and KC in lung homogenates from wild-type, caspase-2 KO and caspase-4 KO mice infected with Sma. For multiple group comparisons Kruskal-Wallis test with Dunn’s multiple-comparison post-test was used: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. For in vitro experiments averaged data from >3 separate experiments are shown.

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