Figure 2
From: Proteomic analysis at the sites of clinical infection with invasive Streptococcus pyogenes
Immunoblotting of clinical fluid samples with CTAbs. The expression of PGK, enolase, SIC, and SpeB in fluid samples in clinical fluid samples was confirmed using antibodies against each of these antigens. Clinical fluid samples F2 and F8 along with corresponding cultured supernatant samples, S2 and S8, were prepared and analysed as described in the Methods section along with purified preparation of SIC and SpeB. The blots were developed with both a CTAb antiserum and corresponding pre-immune serum in each case to ensure that overdevelopment was avoided and to provide evidence that the observed immunoreactivity was antigen-specific. Blots developed using different antibodies are shown as clearly separate panels surrounded by white space; full length images of relevant lanes are shown.
