Figure 1 | Scientific Reports

Figure 1

From: Hedgehog Interacting Protein Promotes Fibrosis and Apoptosis in Glomerular Endothelial Cells in Murine Diabetes

Figure 1

Hyperglycemia-induced renal Hhip expression in vivo (Akita (a–c) and db/db mice (d–f) at the age of 20 weeks) and in vitro (mECs (g) and mPODs (h)). (a,d) qPCR of Hhip mRNA expression in renal cortex. Hhip mRNA expression were normalized by their corresponding β-actin mRNA. (b,e) WB analysis of Hhip, Shh, TGFβ1 and TGFβ1RII in renal cortex. **P ≤ 0.01; ***P ≤ 0.001 vs. WT or db/m; Values represent the mean ± SEM. (c,f) Hhip- and TGFβ1-IHC in the kidneys (scale bar, 50 µm). (g–h) IF staining in mECs (g) and mPODs (h) (scale bar, 50 µm).

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