Table 1 Primers sequence from 5′ to 3′ used in this study.
Oligo Name | Sequence (5′->3′) |
|---|---|
MSR-PCR: | |
HIF-3α amplicon Fw #1 | CCGCCCCCATCCTCTCCCC |
HIF-3α amplicon Rw #1 | CCATCGCCCAGGCCCCCG |
HIF-3α amplicon Fw #2 | CCTGGAGACCCCTGAGCTGGATTGT |
HIF-3α amplicon Rw #2 | CCCGCAGAAGCCTGGGGACTGCTCA |
HIF-3α amplicon Fw #3 | CGGATTCACTGAGAAGTGGTTG |
HIF-3α amplicon Rw #3 | CTCACGGAGCTAGAGAACCAT |
HIF-3α amplicon Fw #4 | ACTGCAGATAAGTCAGGGAGGG |
HIF-3α amplicon Rw #4 | AAAGAGAAAAGGAGGACGGGAC |
ChIP qPCR: | |
HIF-3α ChIP Fw #1 | GCTCAACTGGGGTTAGGAAATG |
HIF-3α ChIP Rw #1 | ATGTCACTCCTGAAAAGGAGGC |
HIF-3α ChIP Fw #2 | CCTGGAGACCCCTGAGCTGGATTGT |
HIF-3α ChIP Rw #2 | CCCGCAGAAGCCTGGGGACTGCTCA |
HIF-3α ChIP Fw #3 | TCCAAGCTTTATTTTGGGGAGA |
HIF-3α ChIP Rw #3 | GACAGGGAAAGCTGAGGACCTA |
HIF-3α ChIP Fw #4 | CTGGGTATCACACTCCCTTTCC |
HIF-3α ChIP Rw #4 | CTCACGGAGCTAGAGAACCCAT |
HIF-3α ChIP Fw #5 | AGTAGAACATCCAGAGGGCAGG |
HIF-3α ChIP Rw #5 | TCTTCAGGCTTTTTCTCATCCC |
HIF-3α ChIP Fw #6 | ACTGCAGATAAGTCAGGGAGGG |
HIF-3α ChIP Rw #6 | AAAGAGAAAAGGAGGACGGGAC |
RT-qPCR: | |
HIF-3α Ex 1a Fw | GACTGGCGAGCCATGGCG |
HIF-3α Ex 1b Fw | GTGCGCACCCACTCGTAACTCG |
HIF-3α1 Ex 1c Fw | CGCCACAGAGAGGAGCGAGG |
HIF-3α Ex 2 Rw | CACCTGGACAAGGCCTCTAT |
