Figure 1
From: Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA
Bisulfite sequencing of cytochrome b gene region of ESC mtDNA. Three independent preparations of embryonic stem cell (ESC) mtDNA (ESC preps. i–iii) were examined by bisulfite sequencing. After bisulfite conversion, L and H strands of the cytochrome b gene region were PCR-amplified and cloned into T-vectors. Average unconversion rates of cytosines at CG sequences (CG) and those at non-CG sequences [CH (H = A, G and T)] in the cloned fragments were obtained from sequencing data for inserts of each plasmid clone, and means of average rates were calculated with SEM for L strands (L) and H strands (H). Data are presented as percentages. A region of control λDNA−mC was also analysed to confirm conversion efficiency of the reactions (λ). n indicates the number of clones analysed in each category.
