Figure 5
From: Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons
Neurocan impairs ephrin-A5-mediated clustering of NCAM and EphA3 in cortical interneurons in culture. (A) Cortical neuron cultures were pretreated with no neurocan (control) or neurocan followed by preclustered Fc or ephrin-A5-Fc, and localization of endogenous NCAM (green) and EphA3 (red) was assessed in axons of GABA immunopositive axons by confocal microscopy. Scale bars = 5 μM. (B) Pearson’s Correlation Coefficients (R-Total) were generated for each condition using ImageJ co-localization software (n = 3, two-way ANOVA with Bonferonni post-hoc testing, *p < 0.05).
