Figure 5

Effect of prednisolone and vamorolone on quantitative hepatic necrosis. Box plots show median, interquartile range, whiskers show 10^th and 90^th percentiles and points reflect 5^th and 95^th percentiles of quantitative liver necrosis in all experimental groups according to genotype and treatment. Ve represents vehicle, P prednisolone, and Va vamorolone. (N = 14–20 per each of 9 treatment groups). When comparing the percentage of hepatic necrosis between treatments in homozygous mice, a significant increase in hepatic necrosis was observed in vamorolone treated homozygotes compared to both vehicle- (p = 0.005) and prednisolone-treated (p = 0.008) animals. There was also a trend towards increased liver necrosis in prednisolone-treated homozygotes compared to vehicle-treated mice even though this increase in hepatic necrosis did not reach statistical significance (p = 0.15).