Figure 4

Impaired glucose and lipid metabolism in B6-mt^AKR mice. (a) Glucose and fructosamine levels were determined in plasma samples from random-fed B6 and B6-mt^AKR mice (3 M, females). *P = 0.0106 (fructosamine), **P = 0.0045 (glucose), Mann-Whitney U test. N = 12–13 per strain. AKR = B6-mt^AKR. (b) Oral glucose tolerance test (2 g/kg, p.o.) was performed in young (3 months old) B6 and B6-mt^AKR female mice. ****P < 0.0001, two-way ANOVA. (c) Glycogen content, activities of pyruvate kinase (PK) and those of phosphoenolpyruvic carboxykinase (PEPCK), and the ratio of PK to PEPCK were determined in liver samples obtained from the mice tested in a. Free fatty acids levels were measured in plasma samples obtained from overnight-fasted B6 and B6-mt^AKR (3 M, females, same animals tested in a). *P = 0.0111 (PK), **P = 0.0041 (PK/PEPCK), **P = 0.0027 (FFA), Mann-Whitney U test. (d) Liver lipid metabolites (free carnitine, acylcarnitines, phosphatidylcholines, lysophosphatidylcholines and sphingomyelines) were measured in B6 and B6-mt^AKR. AKR = B6-mt^AKR. Females, n = 5/strain.