Figure 3

Expression of pro-fibrotic genes in remnant kidney of CKD mice with PC-AKI. Gene expression was assessed by qRT-PCR in the kidney at 2, 7, and 28 days of contrast (C) or saline (S) administration. (A) Connective tissue growth factor (Ctgf), (B) Transforming growth factor β (Tgfβ)-1, (C) Tgfβ-2 and (D); collagen-IVa (Col-IVa) at 2, 7, and 28 days in the kidney with contrast (C) or saline (S) administration. Each data point in the line graph represents mean ± SEM of 4–6 animals per group. Two-way ANOVA with Student t test with Bonferroni correction was performed. Significant differences between the respective time points among C and S indicated by * at P < 0.05.