Figure 1

Profiling of the effect of 089. (a) Chemical structure of 089; (b) Fitness defect scores of the (HIP) heterozygous deletion strains treated with 089. FD scores were calculated for each deletion strain as the difference of the abundance of the strain in the control compared to the treatment. Clearance, used as measure of the drug selectivity, was calculated as the difference of the FD of a given strain and the two closest FDs. Deletion strains having significant positive FD are potential targets of the compound; black triangles indicate the strains having significant FD and high clearance; (c) Effects of several concentrations of 089 on the growth of representative heterozygous strains selected based on the HIP results. The percentage of growth was calculated as the difference of the treated sample’s OD₆₀₀ compared to the control OD₆₀₀ (DMSO- treated deletion strain). Vertical bars indicate the standard deviation of three independent biological replicates. *Wilcoxon test p < 0.05 between the control and the strain indicated by the asterisk color, corresponding to the legend.