Figure 1
Change in serum total IgE levels of mice sensitized with DNFB according to Protocol 1. (a) Protocol 1 is a conventional protocol for hapten-induced dermatitis. It involves five DNFB sensitizations at weekly intervals. (b and c) The dorsal skin of BALB/c mice was painted with 100 µL of 0.15% DNFB in vehicle [acetone/olive oil (3:1)] (black circle of b, n = 3 and c, n = 8) or 100 µL of vehicle (white circle of b, n = 3) every week for 4 weeks. Blood samples were collected from the legs at the indicated time points. The serum IgE concentrations were measured by sandwich ELISA. (b) and (c) indicates different mice. The individual data of the three mice that underwent painting with DNFB and vehicle, respectively, are shown (b). (d) The frequency of scratching behavior of three mice per group is indicated. The movie was recorded for 15 min after 5th DNFB treatment. (c,d) The distributions of IgE and scratching frequency are represented as a box plot together with the medium and inter quarter range (IQR). *P < 0.05 and **P < 0.01, versus IgE value of the pre-treatment. The x-axis is not drawn to scale.
