Figure 4
Gross morphology and histology of the dorsal skin of mice that underwent Protocol 5. (a) Photos of the dorsal skin were taken before and 1, 2, 3, and 4 days after the second DNFB painting session. For comparison, the dorsal skin of a control mouse that was painted with vehicle was photographed 4 days after the second vehicle application. (b and c) Hematoxylin and eosin staining (b) and toluidine blue staining (c) of the dorsal skin samples taken before and 1, 2, 3, 4 and 5 days after the second DNFB painting session. The arrows indicate skin lesions, such as oedema and lymphocytes, and the arrowheads indicate mast cells. Magnification, ×100; scale bar = 100 μm. (d) Epidermal thickness was measured from H&E stained images (n = 5, Mean and s.d.). (e) Infiltration of mast cells into dermal and subcutaneous tissue was observed and counted (n = 4, DNFB 1 day, n = 5, vehicle and n = 6, DNFB 5 day, Mean and s.d.). (f) Lymphocyte accumulation in subcutaneous tissue was observed and counted (n = 5, Mean and s.d.). **P < 0.01, versus vehicle-treated mice.
