Figure 5
Expression of inflammatory cytokines and AD-related gene in the painted skin before and 24 h after the second painting session in Protocol 5. Total RNA was extracted from the dorsal skin of the Protocol 5-treated mice before (0 h) and 24 h after the second DNFB painting sessions. Quantitative PCR was performed to determine the mRNA expression of IL-4, IL-6, IL-10, IL-17A, IL-1β, IFN-γ, filaggrin, and TNF-α after second painting. Mean and s.d. of three mice per group.
