Figure 2
From: Detection of functional protein domains by unbiased genome-wide forward genetic screening
Screening for camptothecin resistance highlights TOP1 and its functional domains. (a) Genes found mutated in screenings for camptothecin resistance sorted by the number of non-synonymous, independent mutations identified in each gene. (b) Fraction of strains identified as mutated in TOP1 by initial analysis (light yellow) and upon manual inspection of the TOP1 gene (dark yellow); blue represents clones where no TOP1 mutation was identified. (c) Mutation types identified in EMS mutagenized samples and non-EMS mutagenised samples. Location of nonsense (d), frameshift (e) and missense (f) mutations in the TOP1 gene with respect to the primary protein sequence. Mutations identified in EMS-treated samples are colored red, while those from non-mutagenized samples are colored green.
