Figure 1
APOBEC3 gene expression in human airway epithelium (HAE) cell culture. APOBEC3 gene expression was analysed in 3D HAE cultures. mRNA levels were evaluated by quantitative RT-PCR. Expression was assessed (a) in uninfected HAE cultures, (b) in cultures infected with human coronavirus (HCoV)-NL63 and (c) in cultures infected with influenza A virus (IVA) strain H3N2. Results are presented as mRNA copy number per ml. Infection was performed at 400 TCID50 per ml, for 4 days at 32 °C (for HCoV-NL63) or for 2 days at 37 °C (for IVA). Each experiment was performed at least three times with clinical material from different donors, and with two technical replicates. For comparisons by Student’s t-test, *Indicates P < 0.05; **Indicates P < 0.005.
