Table 1 Primers and probes used for a RT-qPCR.
Target | Primer | Primer sequence (5′–3′) |
|---|---|---|
HCoV-NL63 | Sense | CTG TGG AAA ACC TTT GGC ATC |
Antisense | CTG TGG AAA ACC TTT GGC ATC | |
Probe | ATG TTA TTC AGT GCT TTG GTC CTC GTG AT | |
A3A | Sense | TGG TTC CTT CTT TGC AGT TGG ACC |
Antisense | GCA GCA TTT GCA GTG CCT CCT TAT | |
A3B | Sense | AGC ACA TGG GCT TTC TAT GCA ACG |
Antisense | AGG AGA TGA ACC AAG TGA CCC TGT | |
A3C | Sense | AAC GAA ACT TGG CTG TGC TTC ACC |
Antisense | AGA CAG TAT GTC GTC GCA GAA CCA | |
A3D | Sense | AGG CAG GAG GTG TAT TTC CGG TTT |
Antisense | GGT GCT CAG CCA AGA ATT TGG TCA | |
A3F | Sense | TCC GTG GAG ATC ATG GGC TAC AAA |
Antisense | TGC AGC TTG CTG TCC AGG AAT AGA | |
A3G | Sense | GCT GTG CCC AGG AAA TGG CTA AAT |
Antisense | ACA AAG GTG TCC CAG CAG TGC TTA | |
A3H | Sense | TGA CTT CAT CAA GGC TCA CGA CCA |
Antisense | GTC AGC AAA CTT TGG GAA GCC CAT | |
TBP | Sense | CCC ATG ACT CCC ATG ACC |
Antisense | TTT ACA ACC AAG ATT CAC TGT GG |
