Figure 2

Binding of IgG-scTRAIL fusion proteins. (a) Combined binding of affinity-purified hu225 IgG-scTRAIL fusion protein variants to cell surface-expressed EGFR and TRAIL receptors was analysed on Colo205 and HCT116 cells, together with EGFR binding of sole hu225 IgG as a reference (n = 3, mean ± S.D.). (b) The binding of HC-scTRAIL at three concentrations was analysed on Colo205 cells in absence or presence of a 200-fold molar excess of either cetuximab or TRAILR2-Fc for competition (n = 1, mean of triplicates ± S.D.). (c) The binding of hu225 IgG-scTRAIL fusion protein variants and hu225 IgG to EGFR-Fc was determined by ELISA (n = 3, mean ± S.D.). (d) The binding of scTRAIL fusion protein variants, hu225 IgG and Fc-scTRAIL-FAVSGAA to TRAILR1-Fc and TRAILR2-Fc was analysed by ELISA (n = 3, mean ± S.D.).