Figure 3
From: IgG-single-chain TRAIL fusion proteins for tumour therapy
Cell death induction of IgG-scTRAIL proteins in vitro. The cell death inducing activity of hu225 IgG-scTRAIL fusion protein variants and Fc-scTRAIL-FAVSGAA in Colo205, HCT116 and WM1366 tumour cell lines was analysed in vitro by cell viability assays. Tumour cells were incubated with the proteins titrated in triplicates for 16 h, followed by crystal violet staining. For competition of EGFR targeting, the assay was performed likewise, but IgG-scTRAIL fusion proteins were co-incubated with 70 nM cetuximab, which was added 30 min prior to addition of the scTRAIL proteins (n = 3, mean ± S.D.).
