Table 2 EC50 values of protein binding to EGFR-positive tumour cells (flow cytometry), EGFR-Fc, TRAILR1-Fc and TRAILR2-Fc (ELISA), n = 3, mean ± SD.
From: IgG-single-chain TRAIL fusion proteins for tumour therapy
Molecule | Colo205 (pM) | HCT116 (pM) | EGFR-Fc (pM) | TRAILR1-Fc (nM)* | TRAILR2-Fc (nM)* |
|---|---|---|---|---|---|
hu225 IgG | 60 ± 41 | 70 ± 21 | 156 ± 19 | — | — |
LC-scTRAIL | 148 ± 83 | 181 ± 79 | 460 ± 27 | 8.0 ± 1.5 | 0.9 ± 0.1 |
HC-scTRAIL | 81 ± 59 | 118 ± 33 | 343 ± 72 | 3.3 ± 0.6 | 0.8 ± 0.1 |
LC/HC-scTRAIL | 146 ± 28 | 91 ± 72 | 315 ± 87 | 12.0 ± 1.6 | 3.4 ± 0.5 |
Fc-scTRAIL-FAVSGAA | n. d. | n. d. | — | 7.3 ± 0.8 | 1.1 ± 0.1 |
hu225 IgG# | 28 ± 6 | 8 ± 3 | — | — | — |
Cetuximab# | 19 ± 8 | 9 ± 3 | — | — | — |
