Table 3 EC50 values (pM scTRAIL units) of protein bioactivity on EGFR-positive tumour cells (n = 3, mean ± S. D.).
From: IgG-single-chain TRAIL fusion proteins for tumour therapy
Colo205 w/o BZB | Colo205 + BZB | HCT116 w/o BZB | HCT116 + BZB | WM1366 w/o BZB | WM1366 + BZB | |
|---|---|---|---|---|---|---|
Fc-scTRAIL-FAVSGAA | 508 ± 11 | 46.1 ± 0.4 | 713 ± 134 | 198 ± 19 | 57.6 ± 18.4 | 6.1 ± 2.1 |
HC-scTRAIL | 58.5 ± 1.5 | 16.2 ± 1.4 | 31.5 ± 2.9 | 24.2 ± 1.5 | 4.7 ± 2.2 | 1.3 ± 0.6 |
HC-scTRAIL + Cetuximab | 397 ± 7 | 48.1 ± 2.7 | 525 ± 62 | 244 ± 15 | 74.7 ± 28.6 | 13.6 ± 6.2 |
LC-scTRAIL | 92.3 ± 2.4 | 21.5 ± 1.7 | 36.3 ± 1.4 | 29.4 ± 9.5 | 4.5 ± 1.1 | 1.6 ± 0.4 |
LC-scTRAIL + Cetuximab | 422 ± 56 | 62.3 ± 2.4 | 467 ± 51 | 199 ± 67 | 62.9 ± 17.1 | 11.3 ± 2.7 |
LC/HC-scTRAIL | 30.5 ± 0.9 | 5.6 ± 0.9 | 7.5 ± 0.4 | 5.7 ± 2.3 | 1.2 ± 0.4 | 0.3 ± 0.1 |
LC/HC-scTRAIL + Cetuximab | 54.1 ± 6.1 | 8.8 ± 1.3 | 47.7 ± 13.0 | 23.5 ± 8.4 | 13.1 ± 5.0 | 2.7 ± 0.9 |
