Figure 3
From: Cystoid edema, neovascularization and inflammatory processes in the murine Norrin-deficient retina
Activation of glial cells in the adult Ndphy/− retina at two months of age. (A–D) retinal whole mount preparations labelled for the detection of retinal vasculature with isolectin in WT (A) and mutant (B) animals. GFAP staining revealed large quantities of astrocytes covering the retina of Ndphy/− mice (D) compared to control (C). (E,F) retinal cryosections stained for GFAP revealed extensive gliosis and activated Müller cells that span the entire retina of mutant animals (F) whereas in WT animals gliosis were not observed (E). GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer, PC: photoreceptors, RPE: retinal pigment epithelium. Scale bar, 50 µm. Cells of the mononuclear phagocyte system accumulate in the extravascular space in the retina of 2-months-old Ndphy/− mice. (G) FITC-stained retinal whole mount overview, (H) magnification. (I) retinal digest preparation. Some cells that were present on the FITC-stained retinal whole mounts (arrows and rings) disappeared after retinal digestion (rings) whereas others were still recognizable (arrows), thus indicating that cells of the mononuclear phagocyte system moved outside the vascular compartment to be secondarily eliminated by the retinal digestion step. A: artery, V: vein.
