Figure 4

(a) Effect of metal ions and EDTA on AiiK activity. (b) Effect of zinc on AiiK activity in vitro. All experiments were carried out in triplicates. (PC stands for positive control, containing 4 μg/mL AiiK, 50 μM C10-HSL, and 10 mM PBS (pH 7.4), then the reaction was performed at 45 °C for 10 min).