Table 1 Kinetic constants of AiiK for degrading AHLs.

From: Characterization of AiiK, an AHL lactonase, from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1

AHL

Mean ± SD

k cat /K m (s−1·mM−1)

k cat (s−1)

K m (mM−1)

C6-HSL

0.649 ± 0.069

0.204 ± 0.031

3.2

3-Oxo-C6-HSL

0.168 ± 0.026

0.066 ± 0.020

2.7

C10-HSL

1.321 ± 0.214

0.315 ± 0.065

3.9

C14-HSL

0.038 ± 0.002

0.036 ± 0.004

0.92

  1. Note: Reactions were performed at pH 7.4 and 25 °C. And all data in this study were exbihited as mean ± the standard deviation (SD).
Back to article page