Figure 1 | Scientific Reports

Figure 1

From: Simultaneous detection of eight avian influenza A virus subtypes by multiplex reverse transcription-PCR using a GeXP analyser

Figure 1

Specificity analyses of GeXP detection of eight avian influenza A viral subtypes with multiplex primers. Cy5-labelled PCR products were separated via GeXP capillary electrophoresis and detected by fluorescence spectrophotometry, given as dye signals in arbitrary units on the y-axis. Each peak was identified by comparing the expected to the actual PCR product size on the x-axis. Panels (A-I and K) show the individual target gene amplification results for M, H1, H2, H3, H5, H6, H7, H9, H10 and all amplified genes simultaneously, respectively. RNase-free water was used as the negative control (J). Red peaks indicate the DNA size standard.

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