Table 1 GeXP primers designed to detect eight subtypes of the avian influenza A virus.

From: Simultaneous detection of eight avian influenza A virus subtypes by multiplex reverse transcription-PCR using a GeXP analyser

Primer

Forward primer (5 → 3)a

Reverse primer (5 → 3)a

Size (bp)

M

AGGTGACACTATAGAATAAGCCGAGATCGCGCAGA

GTACGACTCACTATAGGGACGCTCACTGGGCACGGT

192

H1

AGGTGACACTATAGAATACCAGAAYGTGCATCCTATCACT

GTACGACTCACTATAGGGATATCATTCCTGTCCAWCCCCCT

198

H2

AGGTGACACTATAGAATATTCGAGAAAGTRAAGATTYTGCC

GTACGACTCACTATAGGGACCAGACCATGTTCCTGAAGAA

152

H3

AGGTGACACTATAGAATATTGCCATATCATGYTTTTTGCTTTG

GTACGACTCACTATAGGGAAATGCAAATGTTGCACCTAATGTTG

131

H5

AGGTGACACTATAGAATAGGAAAGTGTAAGAAACGGAACGTA

GTACGACTCACTATAGGGA CACATCCATAAAGAYAGACCAGC

223

H6

AGGTGACACTATAGAATA TCTCAAACAAGGCCCCTCTC

GTACGACTCACTATAGGGA TCCCATTTCGGGCATTAGGC

173

H7

AGGTGACACTATAGAATAAGAATACAGATTGACCCAGTSAA

GTACGACTCACTATAGGGA CCCATTGCAATGGCHAGAAG

142

H9

AGGTGACACTATAGAATAACCATTTATTCGACTGTCGCCT

GTACGACTCACTATAGGGACATTGGACATGGCCCAGAA

118

H10

AGGTGACACTATAGAATA AACACGGACACRGCTGA

GTACGACTCACTATAGGGA ATTGTTCTGGTAWGTGGAAC

167

Cy5-Tag-F b

AGGTGACACTATAGAATA

  

Tag-R

GTACGACTCACTATAGGGA

  
  1. Underlined oligonucleotides are universal sequences.
  2. aDegenerate nucleotide abbreviations are as follows: R, A/G; W, A/T; Y, C/T.
  3. bThe primer is contained in the PCR primer mix.
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