Table 1 SPR analysis of NHBA-glycan interactions.

From: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein

Glycan

KD (μM) (mean ± standard deviation)

Index

Name

Structure

46

β-Glc6P

6-H2PO3Glcβ-sp4

0.056 ± 0.025

2E

P1 antigen

Galα1-4Galβ1-4GlcNAc

14.93 ± 3.48

8A

Sulfo Lewis A

SO3-3Galβ1-3(Fucα1-4) GlcNAc

33.4 ± 2.54

12B

Neocarratetraose-41-O-sulfate (Na+)

C24H37O22SNa

0.44613 ± 0.1511

12C

Neocarrahexaose-24,41,3,5-tetra-O-sulfate (Na+)

C36H52O40S4Na4

NCDB

13E

Hyaluronan disaccharide ΔDi-HA

GlcA/IdoA -GlcNAc

0.13 ± 0.03

17M

GT3 ganglioside sugar

Neu5Acα2-8Neu5Acβ2-8Neu5Acα2-3Galβ1-4Glc

0.21 ± 0.056

13K

Chondroitin sulfate

(GlcA/IdoAβ1-3(±4/6S) GalNAcβ1-4) n (n < 250)

0.0052 ± 0.0024

14J

Heparan sulfate

(GlcA/IdoAα/β1-4GlcNAcα1-4(±NS)) n (n = ~200)

1.362 ± 0.200

13J

Heparin

(GlcA/IdoAα/β1-4GlcNAcα1-4) n (n = ~200)

0.052 ± 0.026

  1. NCDB: No concentration dependent binding observed within the range of the instrument’s detection.