Figure 3 | Scientific Reports

Figure 3

From: The nanos1 gene was duplicated in early Vertebrates and the two paralogs show different gonadal expression profiles in a shark

Figure 3

nanos1A and nanos1B transcripts show distinct tissue distributions in male and female S. canicula. Messenger RNA relative abundances of nanos1A (A,B) and nanos1B (C,D) were quantified in panels of tissues in male (A,C) and female (B,D) dogfish by real-time PCR. Data were normalized using 5S rRNA. In both sexes, nanos1A showed a preferential expression in brain (A,B) whereas nanos1B showed a relatively ubiquitous expression pattern (C,D). Mature ovaries sampled from adult females and containing vitellogenic oocytes showed no significant difference of expression levels compared to immature ovaries sampled from pre-adult females and containing previtellogenic oocytes only (B,D). Data are presented as mean + SD (N = 3). Differences in gene expression were evaluated using one-way analysis of variance followed up with Games-Howell test. Significant differences (p ≤ 0.05) in gene expression compared to the testicular zone A0 or the immature ovary are indicated by asterisks (*).

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